91久久精品美女高潮喷,亚洲一区无码精品色提供亚洲,日韩专区亚洲专区欧美专区,国产熟女高潮免费视频

免費咨詢熱線:
15522676233
技術(shù)文章
首頁 > 技術(shù)中心 > PEI25K轉(zhuǎn)染試劑的應(yīng)用

PEI25K轉(zhuǎn)染試劑的應(yīng)用

 更新時間:2022-11-24 點擊量:967

PEI又稱為線性化聚乙烯亞胺,是人工合成的含有高密度正電荷的有機大分子陽離子聚合物。PEIDNA通過正負電荷的相互吸引形成復(fù)合物,這些復(fù)合物很容易通過內(nèi)吞作用進入細胞。PEI-DNA復(fù)合物能很好地抵御溶酶體的降解作用,有效提高轉(zhuǎn)染效率。PEI可作用于大至135000個核苷酸的質(zhì)粒,所以較大的質(zhì)粒也可以成功轉(zhuǎn)染。目前市面上PEI轉(zhuǎn)染試劑有很多種,PolysciencesPEI線性轉(zhuǎn)染試劑適用于多種真核細胞,用途廣泛。

一、Polysciences線性PEI轉(zhuǎn)染試劑(PEI 25K)特性:

貨號:23966-1

分子式:(CH2CH2NH)n

分子量:25000

外觀:白色至黃色粉末

熔點:73-75

溶解性:溶于:熱水、低pH值的冷水、甲醇和乙醇;

不溶于:苯、YI醚和丙酮。

微信截圖_20221123145305.png

二、Polysciences聚乙烯亞胺轉(zhuǎn)染試劑(PEI 25K)的應(yīng)用:

遞送物質(zhì)
轉(zhuǎn)染細胞類型轉(zhuǎn)染細胞舉例(包括但不限于)
DNA貼壁/懸浮細胞HEK293
CHO
HELA
SF9昆蟲細胞
SF21昆蟲細胞等真核細胞系
三、應(yīng)用Polysciences PEI線性轉(zhuǎn)染試劑發(fā)表的高分文章:

(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9.  IF19.160

試劑:PEI 25K;應(yīng)用:DNA轉(zhuǎn)染人胚腎293S細胞。

(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54.  IF10.633

試劑:PEI 25K;應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染小鼠骨髓間充質(zhì)干細胞(D1)。

(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020.  IF6.630

試劑:PEI 25K;應(yīng)用:包裝質(zhì)粒pCMVdelta8.9、包膜質(zhì)粒VSV-GPD-1-GFP、PD-L1-GFP或空GFP sFFV轉(zhuǎn)移質(zhì)粒轉(zhuǎn)染HEK293T細胞

(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensormodified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276.  IF4.395

試劑:PEI 25K;應(yīng)用:轉(zhuǎn)染HEK293T細胞。

(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739.  IF:4.345

試劑:PEI 25K(23966-1);應(yīng)用:質(zhì)粒轉(zhuǎn)染HEK293FT細胞

(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373.  IF3.602

試劑:PEI 25K(23966-1);應(yīng)用:shRNA-PLKO.1質(zhì)粒轉(zhuǎn)染HEK293FT細胞。

(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132.  IF3.595

試劑:PEI 25K應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染懸浮生長的CHO DG44細胞。

(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.

試劑:PEI 25K應(yīng)用:DNA轉(zhuǎn)染HEK293T細胞。

(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.  

試劑:PEI 25K;應(yīng)用:將編碼fLuc基因的DNA遞送至小鼠。

(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.  

試劑:PEI MAX(24765)應(yīng)用:用質(zhì)粒DNA轉(zhuǎn)染ET801載體—幼倉鼠腎來源細胞。

北京百奧創(chuàng)新科技有限公司提供多款Polysciences轉(zhuǎn)染試劑,具體信息請咨詢百奧創(chuàng)新客服。(Polysciences PEI 25K 23966-1現(xiàn)貨打折,*!機會有限,不要錯過!)

微信截圖_20221123150211.png



醴陵市| 镇沅| 青川县| 建德市| 确山县| 凤冈县| 宁津县| 商丘市| 陆河县| 安多县| 马边| 开阳县| 拜泉县| 巴南区| 民丰县| 高邮市| 辽中县| 郎溪县| 涿州市| 天祝| 清水县| 共和县| 绥阳县| 巴林右旗| 石家庄市| 平凉市| 颍上县| 黑山县| 五莲县| 华宁县| 囊谦县| 视频| 平武县| 航空| 习水县| 潞城市| 丰都县| 衡山县| 长丰县| 黎川县| 镇雄县|